A VERTEBRATE ACTIN-RELATED PROTEIN IS A COMPONENT OF A MULTISUBUNIT COMPLEX INVOLVED IN MICROTUBULE-BASED VESICLE MOTILITY

被引：164

作者：

LEESMILLER, JP ^{[1
]}

HELFMAN, DM ^{[1
]}

SCHROER, TA ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

来源：

NATURE | 1992年 / 359卷 / 6392期

关键词：

D O I：

10.1038/359244a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

ACTIN is a cytoskeletal protein which is highly conserved across eukaryotic phyla. Actin filaments, in association with a family of myosin motor proteins, are required for cellular motile processes as diverse as vesicle transport, cell locomotion and cytokinesis1,2. Many organisms have several closely related actin isoforms3,4. In addition to conventional actins, yeasts contain actin-related proteins that are essential for viability5,6. We show here that vertebrates also contain an actin-related protein (actin-RPV). Actin-RPV is a major component of the dynactin complex, an activator of dynein-driven vesicle movement7,8, indicating that unlike conventional actins which work in conjunction with myosin motors, actin-RPV may be involved in cytoplasmic movements via a microtubule-based system.

引用

页码：244 / 246

页数：3

共 27 条

[1] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT
ADAMS, MD
KELLEY, JM
GOCAYNE, JD
DUBNICK, M
POLYMEROPOULOS, MH
XIAO, H
MERRIL, CR
WU, A
OLDE, B
MORENO, RF
KERLAVAGE, AR
MCCOMBIE, WR
VENTER, JC
[J]. SCIENCE, 1991, 252 (5013) : 1651 - 1656
[2] CHENEY R E, 1992, Current Opinion in Cell Biology, V4, P27, DOI 10.1016/0955-0674(92)90055-H
[3] CLEVELAND DW, 1983, METHOD ENZYMOL, V96, P222
[4] NUCLEOTIDE-SEQUENCE OF THE HUMAN GAMMA-CYTOSKELETAL ACTIN MESSENGER-RNA - ANOMALOUS EVOLUTION OF VERTEBRATE NONMUSCLE ACTIN GENES
ERBA, HP
GUNNING, P
KEDES, L
[J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (13) : 5275 - 5294
[5] DYNACTIN, A CONSERVED, UBIQUITOUSLY EXPRESSED COMPONENT OF AN ACTIVATOR OF VESICLE MOTILITY MEDIATED BY CYTOPLASMIC DYNEIN
GILL, SR
SCHROER, TA
SZILAK, I
STEUER, ER
SHEETZ, MP
CLEVELAND, DW
[J]. JOURNAL OF CELL BIOLOGY, 1991, 115 (06) : 1639 - 1650
[6] HARLOW E, 1988, ANTIBODIES LABORATOR
[7] ATOMIC MODEL OF THE ACTIN FILAMENT
HOLMES, KC
POPP, D
GEBHARD, W
KABSCH, W
[J]. NATURE, 1990, 347 (6288) : 44 - 49
[8] SITE-DIRECTED MUTAGENESIS OF THE YEAST ACTIN GENE - A TEST FOR ACTIN FUNCTION INVIVO
JOHANNES, FJ
GALLWITZ, D
[J]. EMBO JOURNAL, 1991, 10 (12) : 3951 - 3958
[9] ATOMIC-STRUCTURE OF THE ACTIN - DNASE-I COMPLEX
KABSCH, W
MANNHERZ, HG
SUCK, D
PAI, EF
HOLMES, KC
[J]. NATURE, 1990, 347 (6288) : 37 - 44
[10] EXPRESSION AND CHROMOSOME LOCALIZATION OF THE MURINE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR
KELLEY, KA
STAMM, S
KOZAK, CA
[J]. GENOMICS, 1992, 13 (02) : 381 - 388

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