TRANSCRIPTION OF THE HEAT-SHOCK 70 LOCUS IN TRYPANOSOMA-BRUCEI

被引：34

作者：

LEE, MGS ^{[1
]}

VANDERPLOEG, LHT ^{[1
]}

机构：

[1] COLUMBIA UNIV,DEPT GENET & DEV,NEW YORK,NY 10027

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1990年 / 41卷 / 02期

关键词：

Heat shock protein; Mini-exon; Polycistronic transcription; Post-transcriptional control; Trans-splicing;

D O I：

10.1016/0166-6851(90)90185-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 23-kb heat shock 70 locus of the protozoan parasite Trypanosoma brucei encodes several tightly clustered genes. From 5′ to 3′, a cognate hsp70 gene (gene 1) (see preceding paper, Lee et al.) is separated from a cluster of five identical hsp70 genes (genes 2-6) by about 6 kb of DNA that encodes several RNAs of unknown function. Polycistronic transcripts could be detected in the tandem array of hsp70 genes 2-6 and the maturation of the hsp70 pre-mRNAs involves addition of mini-exons on at least two different alternate 3′ splice acceptor sites. Potential heat shock transcription factor binding sites are present upstream of hsp70 gene 2 and in the intergenic regions of hsp70 genes 2-6, but could not be found upstream of the cognate hsp70 gene 1. © 1990.

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页码：221 / 231

页数：11

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