YEAST RIBOSOMAL-PROTEINS .3. ISOLATION AND CHARACTERIZATION OF 23 RIBOSOMAL-PROTEINS FROM LARGE SUBUNITS OF YEAST

被引：20

作者：

ITOH, T ^{[1
]}

HIGO, K ^{[1
]}

OTAKA, E ^{[1
]}

机构：

[1] HIROSHIMA UNIV,NUCL MED & BIOL RES INST,DEPT BIOCHEM & BIOPHYS,HIROSHIMA 734,JAPAN

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 26期

关键词：

D O I：

10.1021/bi00593a007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proteins of large ribosomal subunits from Saccharomyces cerevisiae were separated into 25 fractions by chromatography on columns of carboxymethylcellulose (CMC). Twenty-three proteins were then purified from the 12 CMC fractions by filtration through Sephadex G-75, Sephadex G-100, and Sephacryl S-200, and/or by phospho- cellulose column chromatography. The isolated proteins are YP 1, YP 2, YP 9, YP 11, YP 13', YP 16, YP 18, YP 26, YP 39, YP 41, YP 42, YP 42', YP 44, YP 45, YP 47', YP 52a, YP 53, YP 55, YP 59, YP 62, YP 68, YP A1, and YP A2. The molecular weight and amino acid composition of these proteins are presented. © 1979, American Chemical Society. All rights reserved.

引用

页码：5787 / 5793

页数：7

共 14 条

[1] COMPARISON OF AMINO-TERMINAL SEQUENCE OF L7-L12-TYPE PROTEINS OF ARTEMIA-SALINA AND SACCHAROMYCES-CEREVISIAE [J].

AMONS, R ;

VANAGTHOVEN, A ;

PLUIJMS, W ;

MOLLER, W ;

HIGO, K ;

ITOH, T ;

OSAWA, S .

FEBS LETTERS, 1977, 81 (02) :308-310

[2]

BIELKA H, 1978, INT REV BIOCHEM, V18, P79

[3]

COLLATZ E, 1976, J BIOL CHEM, V251, P4666

[4]

COLLATZ E, 1977, J BIOL CHEM, V252, P9071

[5] YEAST RIBOSOMAL-PROTEINS .2. ISOLATION AND CHARACTERIZATION OF 14-RIBOSOMAL PROTEINS FROM SMALL SUBUNITS OF YEAST [J].

HIGO, K ;

OTAKA, E .

BIOCHEMISTRY, 1979, 18 (19) :4191-4196

[6] PRIMARY STRUCTURE OF PROTEIN-44 FROM LARGE SUBUNIT OF YEAST RIBOSOMES [J].

ITOH, T ;

WITTMANNLIEBOLD, B .

FEBS LETTERS, 1978, 96 (02) :399-402

[7] RIBOSOMAL PROTEINS .7. 2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS FOR FINGERPRINTING OF RIBOSOMAL PROTEINS [J].

KALTSCHM.E ;

WITTMANN, HG .

ANALYTICAL BIOCHEMISTRY, 1970, 36 (02) :401-&

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9] 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS - IMPROVED METHOD FOR RIBOSOMAL-PROTEINS [J].

METS, LJ ;

BOGORAD, L .

ANALYTICAL BIOCHEMISTRY, 1974, 57 (01) :200-210

[10] YEAST RIBOSOMAL-PROTEINS .1. CHARACTERIZATION OF CYTOPLASMIC RIBOSOMAL-PROTEINS BY 2-DIMENSIONAL GEL-ELECTROPHORESIS [J].

OTAKA, E ;

KOBATA, K .

MOLECULAR AND GENERAL GENETICS, 1978, 162 (03) :259-268

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