A METHOD FOR SIMULTANEOUS DETERMINATION OF SOLUBILITY AND TRANSFER-COEFFICIENT OF OXYGEN IN AQUEOUS-MEDIA USING OFF-GAS MASS-SPECTROMETRY

被引：9

作者：

DORRESTEIJN, RC ^{[1
]}

DEGOOIJER, CD ^{[1
]}

TRAMPER, J ^{[1
]}

BEUVERY, EC ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR,DEPT FOOD SCI,FOOD & BIOPROC GRP,6700 EV WAGENINGEN,NETHERLANDS

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1994年 / 43卷 / 02期

关键词：

MASS SPECTROMETRY; OXYGEN-TRANSFER COEFFICIENT; SOLUBILITY OF OXYGEN;

D O I：

10.1002/bit.260430207

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A static method was developed that simultaneously determined the solubility of oxygen and the oxygen-transfer coefficient in a stirred bioreactor. It was based on the static method developed by van Sonsbeek et al. to determine the ka in a liquid-impelled loop reactor. Only physical properties of the liquid were used to determine both parameters using a mass spectrometer. Data about the solubility of oxygen in water are available from the literature. Therefore, the solubility of oxygen in water was used to compare our data with published data. Furthermore, the solubility of oxygen in trypticase soy broth was compared to literature data. No significant deviations between our data and literature data could be observed. Our static method and the commonly applied dynamic method to determine the oxygen-transfer coefficient yielded similar results. The effect of temperature on the oxygen-transfer coefficient could be expressed as the activation energy needed for the transition of oxygen from the gas to the water phase. This was verified using the Arrhenius equation. (C) 1994 John Wiley & Sons, Inc.

引用

页码：149 / 154

页数：6

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