NOVEL MUTATION (V75T) IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE CONFERS RESISTANCE TO 2',3'-DIDEHYDRO-2',3'-DIDEOXYTHYMIDINE IN CELL-CULTURE

被引:122
作者
LACEY, SF [1 ]
LARDER, BA [1 ]
机构
[1] WELLCOME RES LABS,ANTIVIRAL THERAPEUT RES UNIT,BECKENHAM BR3 3BS,KENT,ENGLAND
关键词
D O I
10.1128/AAC.38.6.1428
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have selected a human immunodeficiency virus type 1 (HIV-1) mutant strain with a moderate (sevenfold) level of resistance to the nucleoside analog 2',3'-didehydro-2',3'-dideoxythymidine (D4T or stavudine). After serial passage of the HXB2 strain of HIV-1 in MT4 cells, a novel mutation involving two nucleotide substitutions in codon 75 of the viral reverse transcriptase, altering valine to threonine, was seen. When introduced into a wild-type HIV-1 background by site-directed mutagenesis, the T-75 mutation conferred cross-resistance to the dideoxynucleosides dideoxyinosine and dideoxycytosine as well as to 2',3'-didehydro-2',3'-dideoxycytosine.
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页码:1428 / 1432
页数:5
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