ADP-RIBOSYLATION OF HMG PROTEINS AND ITS MODULATION BY DIFFERENT EFFECTORS IN THE LIVER OF AGING RATS

The in vitro ADP-ribosylation of high mobility group (HMG) non-histone proteins and its modulation by spermine, butyrate, dexamethasone and 3-aminobenzamide were studied in the liver of young (14 weeks) and old (113 weeks) male rats. ADP-ribosylation of HMG 1 was similar in both ages, whereas that of HMG 2 and 14 decreased but HMG 17 increased in the old. HMG 1 was ADP-ribosylated to a greater extent in young but to a lower level in the old by different effectors except spermine which showed no influence in old age. ADP-ribosylation of HMG 2 was stimulated by spermine, butyrate and dexamethasone in old but only by spermine in young rats. Other effectors decreased the ADP-ribosylation of HMG 2 in young. The ADP-ribosylation of HMG 14 was stimulated by spermine in the old but that of HMG 17 was reduced by butyrate in young and by spermine in the old. Dexamethasone decreased the ADP-ribosylation of both HMG 14 and 17 in young, whereas this showed no change in old age. Aminobenzamide inhibited ADP-ribosylation of only HMG 2 in young but all HMGs except HMG 2 in the old. Such alteration in the ADP-ribosylation of HMG proteins may affect various cellular and nuclear functions of rat liver during aging. © 1990.