ANTISENSE CCAAT ENHANCER-BINDING PROTEIN RNA SUPPRESSES COORDINATE GENE-EXPRESSION AND TRIGLYCERIDE ACCUMULATION DURING DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES

被引：308

作者：

LIN, FT ^{[1
]}

LANE, MD ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205

来源：

GENES & DEVELOPMENT | 1992年 / 6卷 / 04期

关键词：

C/EBP; ADIPOCYTE GENES; 3T3-L1; PREADIPOCYTES;

D O I：

10.1101/gad.6.4.533

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA.

引用

页码：533 / 544

页数：12

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