STRUCTURAL DIFFERENCES IN 5'-FLANKING REGIONS OF RAT CYTOCHROME-P-450ALDO AND CYTOCHROME-P-45011-BETA GENES

被引：32

作者：

MUKAI, K ^{[1
]}

IMAI, M ^{[1
]}

SHIMADA, H ^{[1
]}

OKADA, Y ^{[1
]}

OGISHIMA, T ^{[1
]}

ISHIMURA, Y ^{[1
]}

机构：

[1] KEIO UNIV,SCH MED,DEPT BIOCHEM,35 SHINANOMACHI,SHINJUKU KU,TOKYO 160,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)81321-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two rat genomic clones, one for cytochrome P-450aldo and the other for P45011β, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5′-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-45011β gene. S1 nuclease protection assay revealed a single transcription initiation site for the P450aldo gene, while multiple sites were found for the P-45011β gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-45011β genes is due to differences in the sequences of their 5′-flanking regions. © 1991 Academic Press, Inc.

引用

页码：1187 / 1193

页数：7

共 16 条

[11] THE P450 SUPERFAMILY - UPDATED LISTING OF ALL GENES AND RECOMMENDED NOMENCLATURE FOR THE CHROMOSOMAL LOCI [J].

NEBERT, DW ;

NELSON, DR ;

ADESNIK, M ;

COON, MJ ;

ESTABROOK, RW ;

GONZALEZ, FJ ;

GUENGERICH, FP ;

GUNSALUS, IC ;

JOHNSON, EF ;

KEMPER, B ;

LEVIN, W ;

PHILLIPS, IR ;

SATO, R ;

WATERMAN, MR .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (01) :1-13

[12] MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING RAT ADRENAL CYTOCHROME-P-45011-BETA [J].

NONAKA, Y ;

MATSUKAWA, N ;

MOROHASHI, K ;

OMURA, T ;

OGIHARA, T ;

TERAOKA, H ;

OKAMOTO, M .

FEBS LETTERS, 1989, 255 (01) :21-26

[13]

OGISHIMA T, 1991, J BIOL CHEM, V266, P10731

[14]

OGISHIMA T, 1989, J BIOL CHEM, V264, P10935

[15] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[16] REGULATION OF ALDOSTERONE SYNTHASE CYTOCHROME-P-450 IN RAT ADRENALS BY ANGIOTENSIN-II AND POTASSIUM [J].

SHIBATA, H ;

OGISHIMA, T ;

MITANI, F ;

SUZUKI, H ;

MURAKAMI, M ;

SARUTA, T ;

ISHIMURA, Y .

ENDOCRINOLOGY, 1991, 128 (05) :2534-2539

← 1 2 →