PREPARATION AND PROPERTIES OF BETA-GALACTOSIDASE CONFINED IN CELLS OF KLUYVEROMYCES SP

被引：30

作者：

CHAMPLUVIER, B ^{[1
]}

KAMP, B ^{[1
]}

ROUXHET, PG ^{[1
]}

机构：

[1] CATHOLIC UNIV LOUVAIN,UNITE CHIM INTERFACES,PL CROIX SUD 1,B-1348 LOUVAIN LA NEUVE,BELGIUM

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1988年 / 10卷 / 10期

关键词：

Biocatalysts - Cell culture;

D O I：

10.1016/0141-0229(88)90108-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactase (β-D-galactosidase EC 3.2.1.23) was immobilized in yeast cells of the genus Kluyveromyces. Comparison of various solvents or polycations led to the selection of a chloroform-ethanol mixture for cell permeabilization. The lactase was confined inside the ghosts, with a minimum deactivation, by treatment with 0.4% glutaraldehyde. The combined procedure, permeabilization by chloroform-ethanol and confinement by glutaraldehyde, was applied to strain, K. lactis CBS 683, yielding a biocatalyst with an activity of 3.8 units ONP per milligram dry weight as calculated from Vmax. The biocatalyst could be stored in suspension at 4°C for 14 months with 20% deactivation.

引用

页码：611 / 617

页数：7