3-PHOSPHORYLATED AND 4-PHOSPHORYLATED PHOSPHATIDYLINOSITOLS IN THE AQUATIC PLANT SPIRODELA-POLYRHIZA L

被引:37
作者
BREARLEY, CA
HANKE, DE
机构
[1] Botany School, University of Cambridge, Cambridge CB2 3EA, Downing Street
关键词
D O I
10.1042/bj2830255
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Labelling of Spirodela polyrhiza L. plants with [H-3]inositol and [P-32]P(i) yielded a series of phosphoinositides which were identified as PtdIns, PtdIns4P and PtdIns(4,5)P2. In addition, systematic degradation of a phospholipid extract identified PtdIns3P. Analysis of the distribution of P-32 label between the monoester and diester phosphate groups of PtdIns3P and PtdIns4P revealed differences in the labelling of the monoester phosphate, suggesting that the two PtdInsP species are not synthesized or metabolized in a co-ordinate manner.
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页码:255 / 260
页数:6
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