DNA RECOGNITION AND CLEAVAGE BY THE ECOP15 RESTRICTION ENDONUCLEASE

被引：69

作者：

HADI, SM ^{[1
]}

BACHI, B ^{[1
]}

SHEPHERD, JCW ^{[1
]}

YUAN, R ^{[1
]}

INEICHEN, K ^{[1
]}

BICKLE, TA ^{[1
]}

机构：

[1] UNIV BASEL,BIOZENTRUM,CH-4056 BASEL,SWITZERLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 134卷 / 03期

关键词：

D O I：

10.1016/0022-2836(79)90372-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

EcoP15 is a restriction-modification enzyme coded by the P15 plasmid of Escherichia coli. We have determined the sites recognized by this enzyme on pBR322 and simian virus 40 DNA. The enzyme recognizes the sequence: {A figure is presented} In restriction, the enzyme cleaves the DNA 25 to 26 base-pairs 3′ to this sequence to leave single-stranded 5′ protrusions two bases long. © 1979.

引用

页码：655 / 666

页数：12

共 23 条

[11] HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI .X. IN VITRO RESTRICTION OF PHAGE FD REPLICATIVE FORM [J].