EFFECTS OF VASOPRESSIN ON SINGLE CL- CHANNELS IN THE APICAL MEMBRANE OF DISTAL NEPHRON CELLS (A6)

被引:34
作者
MARUNAKA, Y
TOHDA, H
机构
[1] Division of Respiratory Research, The Hospital for Sick Children Research Institute, The University of Toronto Faculty of Medicine, Toronto, Ont. M5G 1X8
关键词
CHLORIDE CHANNEL; PATCH CLAMP; SINGLE CHANNEL CURRENT; CHANNEL NUMBER; KIDNEY;
D O I
10.1016/0005-2736(93)90281-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated how two types of Cl- channels found in sodium transporting epithelium are regulated by arginine vasopressin (AVP). A6 cells cultured on permeable supports for 10 to 14 days have two types of Cl- channels in the apical membrane that have single channel conductances of 3 and 8 pS. In cells without AVP pretreatment, the 3 pS Cl- channel was more frequently observed than the 8 pS Cl- channel. AVP increased the open probability (P-0) and single channel conductance of the 3 pS Cl- channel without significantly changing the P-0 or conductance of the 8 pS Cl- channel. On the other hand, AVP did not affect the number of the 3 pS Cl- channel, but increased the number of 8 pS Cl- channels. These observations suggest that AVP has two different pathways to increase apical membrane chloride conductance in distal nephron A6 cells, i.e., (1) increases the P-0 and single channel conductance of 3 pS Cl- channels and (2) increases the number of 8 pS Cl- channels.
引用
收藏
页码:105 / 110
页数:6
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