SELECTIVE ISOLATION OF AMYLASE DEREPRESSED MUTANTS OF SCHWANNIOMYCES-OCCIDENTALIS

被引：3

作者：

HORN, CH ^{[1
]}

DUPREEZ, JC ^{[1
]}

KILIAN, SG ^{[1
]}

机构：

[1] UNIV ORANGE FREE STATE,DEPT MICROBIOL & BIOCHEM,BLOEMFONTEIN 9300,SOUTH AFRICA

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1991年 / 14卷 / 03期

关键词：

DEREPRESSED MUTANTS; SCHWANNIOMYCES; AMYLOLYTIC YEAST; ALPHA-AMYLASE; GLUCOAMYLASE; PULLULANASE; DEBRANCHING ACTIVITY; AMYLASE;

D O I：

10.1016/S0723-2020(11)80385-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Amylase derepressed mutants of Schwanniomyces occidentalis CSIR-Y993 were obtained with UV light and NTG as mutagens, using a starch medium containing 2-deoxyglucose as repressor for mutant isolation. One outstanding mutant, designated mutant B53, was selected which possessed six-fold higher alpha-amylase and glucoamylase activities, while its debranching activity was five-fold higher than that of the parental strain. Amylase production by this mutant in batch cultures was investigated.

引用

页码：299 / 304

页数：6

共 24 条

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