EFFECT OF POLYVINYL-ALCOHOL ON KINETIC AND THERMAL-PROPERTIES OF BETA-GALACTOSIDASE

被引：8

作者：

ARIGA, O

SANO, T

SANO, Y

机构：

[1] Department of Fine Materials Engineering, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 74卷 / 02期

关键词：

D O I：

10.1016/0922-338X(92)80013-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of PVA (polyvinyl alcohol) with various degrees of polymerization and saponification on the kinetic and thermal characteristics of beta-galactosidase was investigated. With ONPG (2-nitrophenyl-beta-D-galactoside) as a substrate, the enzyme activity increased with increasing PVA concentration. While the influences of the properties of PVA on Michaelis constants were insignificant, the maximum reaction rate of the enzyme increased with increasing degrees of polymerization and saponification. The addition of PVA enhanced the thermal stability of the enzyme, and it increased with increasing PVA concentration. Dependency of the thermal stability on the characteristic properties of PVA was not remarkable.

引用

页码：120 / 122

页数：3

共 15 条

[1] IMMOBILIZATION OF MICROORGANISMS WITH PVA HARDENED BY ITERATIVE FREEZING AND THAWING [J].

ARIGA, O ;

TAKAGI, H ;

NISHIZAWA, H ;

SANO, Y .

JOURNAL OF FERMENTATION TECHNOLOGY, 1987, 65 (06) :651-658

[2] IMMOBILIZATION OF BETA-GALACTOSIDASE WITH POLYVINYL-ALCOHOL [J].

ARIGA, O ;

YAMAKAWA, T ;

FUJIMATSU, H ;

SANO, Y .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1989, 68 (04) :293-295

[3] RELEASE OF THERMOPHILIC ALPHA-AMYLASE FROM TRANSFORMED ESCHERICHIA-COLI BY ADDITION OF GLYCINE [J].

ARIGA, O ;

WATARI, T ;

ANDOH, Y ;

FUJISHITA, Y ;

SANO, Y .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1989, 68 (04) :243-246

[4] INCREASED THERMAL-STABILITY OF BACILLUS-LICHENIFORMIS ALPHA-AMYLASE IN THE PRESENCE OF VARIOUS ADDITIVES [J].

ASTHER, M ;

MEUNIER, JC .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (11) :902-905

[5] GLYCEROL AS AN ENZYME-STABILIZING AGENT - EFFECTS ON ALDEHYDE DEHYDROGENASE [J].

BRADBURY, SL ;

JAKOBY, WB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (09) :2373-&

[6] EFFECT OF ADDITIVES ON THE THERMOSTABILITY OF BACILLUS-STEAROTHERMOPHILUS ALPHA-AMYLASE [J].

BRUMM, PJ ;

TEAGUE, WM .

BIOTECHNOLOGY LETTERS, 1989, 11 (08) :541-544

[7]

GERLSMA SY, 1968, J BIOL CHEM, V243, P957

[8] WATER-SOLUBLE ENZYME-POLYMER GRAFTS - THERMAL STABILIZATION OF GLUCOSE OXIDASE [J].

HIXSON, HF .

BIOTECHNOLOGY AND BIOENGINEERING, 1973, 15 (05) :1011-1016

[9]

JANCSIK V, 1975, J MOL CATAL, V1, P137

[10] STABILIZATION OF ENZYMES AGAINST THERMAL INACTIVATION [J].

KLIBANOV, AM .

ADVANCES IN APPLIED MICROBIOLOGY, 1983, 29 :1-28

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