SIMPLE METHOD TO RECOVER INTACT HIGH MOLECULAR-WEIGHT RNA AND DNA AFTER ELECTROPHORETIC SEPARATION IN LOW GELLING TEMPERATURE AGAROSE GELS

Defined RNA fractions can be recovered from low gelling temperature agarose gels by a combination of agarose melting at 65°C and phenol extraction. By this approach RNA molecules up to a size of 37 kb can be eluted undegraded with a recovery of 60-90%. The method is applicable also to DNA and the eluted DNA can be correctly cleaved with restriction endonucleases as shown for λDNA using EcoRI. © 1979.