SIMPLE METHOD TO RECOVER INTACT HIGH MOLECULAR-WEIGHT RNA AND DNA AFTER ELECTROPHORETIC SEPARATION IN LOW GELLING TEMPERATURE AGAROSE GELS

被引：292

作者：

WIESLANDER, L

机构：

[1] Department of Histology, Karolinska Institutet

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90145-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Defined RNA fractions can be recovered from low gelling temperature agarose gels by a combination of agarose melting at 65°C and phenol extraction. By this approach RNA molecules up to a size of 37 kb can be eluted undegraded with a recovery of 60-90%. The method is applicable also to DNA and the eluted DNA can be correctly cleaved with restriction endonucleases as shown for λDNA using EcoRI. © 1979.

引用

页码：305 / 309

页数：5

共 27 条

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