DIRECT GENE-TRANSFER IN PSOPHOCARPUS-TETRAGONOLOBUS RESISTANCE TO KANAMYCIN

被引：3

作者：

GILL, R

机构：

[1] Plant Biotechnology Section, B.A.R.C., Trombay

来源：

ANNALS OF BOTANY | 1990年 / 66卷 / 01期

关键词：

Naked DNA transformation; Protoplast culture; Psophocarpus tetragonolobus; Regenerated plants; Winged bean;

D O I：

10.1093/oxfordjournals.aob.a087997

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Epicotyl-derived protoplasts of Psophocarpus tetragonolobus were isolated and regenerated to plants. These protoplasts were transformed to kanamycin resistance following uptake of plasmid (pABDl or pHP23) DNA in combination with PEG treatment. Protoplast-derived transformed colonies were selected on kanamycin (75 mg l-1). The transformed calli expressed NPT II activity and also exhibited the presence of the plasmid gene integrated into the plant genome. However, none of the transformed clones showed regeneration of shoot buds. © 1990 Annals of Botany Company.

引用

页码：31 / 39

页数：9

共 35 条

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