MECHANISM OF INTERLEUKIN-1-BETA STIMULATION OF HUMAN AMNION PROSTAGLANDIN BIOSYNTHESIS - MEDIATION VIA A NOVEL INDUCIBLE CYCLOOXYGENASE

被引：85

作者：

MITCHELL, MD ^{[1
]}

EDWIN, SS ^{[1
]}

LUNDINSCHILLER, S ^{[1
]}

SILVER, RM ^{[1
]}

SMOTKIN, D ^{[1
]}

TRAUTMAN, MS ^{[1
]}

机构：

[1] UNIV UTAH,SCH MED,DEPT PEDIAT,SALT LAKE CITY,UT 84132

来源：

PLACENTA | 1993年 / 14卷 / 06期

关键词：

D O I：

10.1016/S0143-4004(05)80379-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have evaluated the mechanism by which interleukin-1β (IL-1β) increases amnion cell PGE2 production in a concentration-dependent manner. IL-1β-stimulated amnion cell PGE2 biosynthesis was time-dependent, and significant stimulation occurred within 2 h of incubation. IL-1β stimulation occurred in the presence of added arachidonic acid but was abrogated by treatment with cycloheximide and actinomycin D. Amnion cells treated with IL-1β recovered rapidly from aspirin pretreatment suggesting an action on fatty acid cyclooxygenase (COX). Increased amounts of COX protein were demonstrated by Western blot analysis within 2 h of IL-1β treatment of amnion cells. Northern blot analysis using a probe specific for a novel form of COX (COX-II) showed an increase in mRNA for this COX within 30 min. This finding using a homologous detection system and human cells of fetal origin in primary culture provides strong support for a physiological role for COX-II in man. © 1993, Baillière Tindall Ltd. All rights reserved.

引用

页码：615 / 625

页数：11

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