MECHANISM OF INTERLEUKIN-1-BETA STIMULATION OF HUMAN AMNION PROSTAGLANDIN BIOSYNTHESIS - MEDIATION VIA A NOVEL INDUCIBLE CYCLOOXYGENASE

被引：85

作者：

MITCHELL, MD ^{[1
]}

EDWIN, SS ^{[1
]}

LUNDINSCHILLER, S ^{[1
]}

SILVER, RM ^{[1
]}

SMOTKIN, D ^{[1
]}

TRAUTMAN, MS ^{[1
]}

机构：

[1] UNIV UTAH,SCH MED,DEPT PEDIAT,SALT LAKE CITY,UT 84132

来源：

PLACENTA | 1993年 / 14卷 / 06期

关键词：

D O I：

10.1016/S0143-4004(05)80379-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have evaluated the mechanism by which interleukin-1β (IL-1β) increases amnion cell PGE2 production in a concentration-dependent manner. IL-1β-stimulated amnion cell PGE2 biosynthesis was time-dependent, and significant stimulation occurred within 2 h of incubation. IL-1β stimulation occurred in the presence of added arachidonic acid but was abrogated by treatment with cycloheximide and actinomycin D. Amnion cells treated with IL-1β recovered rapidly from aspirin pretreatment suggesting an action on fatty acid cyclooxygenase (COX). Increased amounts of COX protein were demonstrated by Western blot analysis within 2 h of IL-1β treatment of amnion cells. Northern blot analysis using a probe specific for a novel form of COX (COX-II) showed an increase in mRNA for this COX within 30 min. This finding using a homologous detection system and human cells of fetal origin in primary culture provides strong support for a physiological role for COX-II in man. © 1993, Baillière Tindall Ltd. All rights reserved.

引用

页码：615 / 625

页数：11

共 34 条

[21] CDNA CLONING AND FUNCTIONAL-ACTIVITY OF A GLUCOCORTICOID-REGULATED INFLAMMATORY CYCLOOXYGENASE [J].

OBANION, MK ;

WINN, VD ;

YOUNG, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4888-4892

[22]

OBANION MK, 1991, J BIOL CHEM, V266, P23261

[23]

OKITA JR, 1983, IN VITRO CELL DEV B, V19, P117

[24] TEMPORAL AND PHARMACOLOGICAL DIVISION OF FIBROBLAST CYCLOOXYGENASE EXPRESSION INTO TRANSCRIPTIONAL AND TRANSLATIONAL PHASES [J].

RAZ, A ;

WYCHE, A ;

NEEDLEMAN, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) :1657-1661

[25]

RAZ A, 1988, J BIOL CHEM, V263, P3022

[26] INTERLEUKIN-1 STIMULATES PROSTAGLANDIN BIOSYNTHESIS BY HUMAN AMNION [J].

ROMERO, R ;

DURUM, S ;

DINARELLO, CA ;

OYARZUN, E ;

HOBBINS, JC ;

MITCHELL, MD .

PROSTAGLANDINS, 1989, 37 (01) :13-22

[27] INFECTION AND LABOR .3. INTERLEUKIN-1 - A SIGNAL FOR THE ONSET OF PARTURITION [J].

ROMERO, R ;

BRODY, DT ;

OYARZUN, E ;

MAZOR, M ;

WU, YK ;

HOBBINS, JC ;

DURUM, SK .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1989, 160 (05) :1117-1123

[28]

ROMERO R, 1990, J REPROD MED, V35, P235

[29]

ROMERO R, 1988, SEMIN PERINATOL, V23, P262

[30] IDENTIFICATION OF A CYCLOOXYGENASE-RELATED GENE AND ITS POTENTIAL ROLE IN PROSTAGLANDIN FORMATION [J].

ROSEN, GD ;

BIRKENMEIER, TM ;

RAZ, A ;

HOLTZMAN, MJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (03) :1358-1365

← 1 2 3 4 →