PURIFICATION AND PROPERTIES OF ALPHA-GLUCOSIDASE FROM SUSPENSION-CULTURED SUGAR-BEET CELLS

被引：7

作者：

YAMASAKI, Y

KONNO, H

机构：

[1] Research Institute for Bioresources, Okayama University, Kurashiki-shi, Okayama

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 09期

关键词：

BETA-VULGARIS; CHENOPODIACEAE; SUGAR-BEET; ALPHA-GLUCOSIDASE;

D O I：

10.1016/S0031-9422(00)98213-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The main alpha-glucosidase from sugar-beet cells has been isolated by a procedure including fractionation with ammonium sulphate, Sephacryl S-200 HR column chromatography, CM-cellulose column chromatography, Mono Q column chromatography, and preparative disc gel electrophoresis. The M(r) of the enzyme was 53 000. The enzyme hydrolysed maltooligosaccharides at a faster rate than maltose. The K(m) values for maltose and maltohexaose were 3.5 and 0.35 mM.

引用

页码：2861 / 2863

页数：3

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