POLYMORPHISM IN 2 PARASITOIDS DETECTED USING RANDOM AMPLIFIED POLYMORPHIC DNA-POLYMERASE CHAIN-REACTION

被引:45
作者
EDWARDS, OR [1 ]
HOY, MA [1 ]
机构
[1] UNIV CALIF BERKELEY, DEPT ENTOMOL SCI, BERKELEY, CA 94720 USA
关键词
TRIOXYS-PALLIDUS; DIGLYPHUS-BEGINI; CHELEX-100; DNA PREPARATION; RAPD; POLYMERASE CHAIN REACTION; GENETIC DIVERSITY; POLYMORPHISM; HYMENOPTERAN GENETICS;
D O I
10.1006/bcon.1993.1034
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We used Chelex 100 chelating resin to prepare DNA for the polymerase chain reaction (PCR) from two species of Hymenopteran parasitoids, Trioxys pallidus and Diglyphus begini. Chelex 100 produces consistent DNA yields for both species, as measured with Hoescht dye fluorometry. Approximately twice as much DNA was obtained from individual D. begini wasps than from T. pallidus wasps, but there were no differences in yield between sexes. We used this DNA to perform random amplified polymorphic DNA (RAPD) analysis, a PCR technique that amplifies various regions of the genome using arbitrarily chosen 10-base primers. Of the 120 primers tested using T. pallidus, 92 produced a total of 342 scorable bands, 118 of which exhibited presence/absence polymorphism. Of the 25 primers tested using D. begini, 18 produced a total of 53 scorable bands, 30 of which exhibited presence/absence polymorphism. The level of genetic variation detected using this technique was greater than any found in Hymenoptera using allozymes. Scorable bands segregated as dominant Mendelian traits. Potential uses of RAPD-PCR in genetic analyses on parasitic Hymenoptera are discussed. © 1993 by Academic Press, Inc.
引用
收藏
页码:243 / 257
页数:15
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