GENERATION OF RABBIT MONOCLONAL-ANTIBODY FRAGMENTS FROM A COMBINATORIAL PHAGE DISPLAY LIBRARY AND THEIR PRODUCTION IN THE YEAST PICHIA-PASTORIS

被引：131

作者：

RIDDER, R

SCHMITZ, R

LEGAY, F

GRAM, H

机构：

[1] SANDOZ PHARMA AG,DEPT DRUG METAB & PHARMACOKINET,CH-4002 BASEL,SWITZERLAND

[2] SANDOZ PHARMA AG,PRECLIN RES,CH-4002 BASEL,SWITZERLAND

来源：

BIO-TECHNOLOGY | 1995年 / 13卷 / 03期

关键词：

D O I：

10.1038/nbt0395-255

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have applied the combinatorial immunoglobulin library and phage display technologies to generate monoclonal rabbit single-chain Fv (scFv) antibody fragments specific for recombinant human leukemia inhibitory factor (rhLIF). The B cell immunoglobulin repertoire of an immunized rabbit was immortalized by the combinatorial cloning of the rearranged variable domains of light (V-I) and heavy (V-H) chains. Affinity selection of the library displaying the rabbit antibody domains on the phage surface resulted in the isolation of phage encoding scFv antibodies which specifically bind to the antigen, We utilized the methylotrophic yeast Pichia pastoris for high level secretion of soluble and functional scFv antibody fragment. More than 100 mg/L of pure and functional rabbit anti-rhLIF scFv antibody was obtained directly from the P. pastoris culture supernatant by one-step affinity chromatography.

引用

收藏

页码：255 / 260

页数：6

相关论文

共 46 条

[1] EVOLUTION OF THE IMMUNOGLOBULIN-K LIGHT CHAIN LOCUS IN THE RABBIT - EVIDENCE FOR DIFFERENTIAL GENE CONVERSION EVENTS [J].

AKIMENKO, MA ;

MARIAME, B ;

ROUGEON, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (14) :5180-5183

[2]

Ausubel FM., 2006, ENZYMATIC MANIPULATI

[3]

Barbas Carlos F. Iii, 1993, Current Opinion in Biotechnology, V4, P526, DOI 10.1016/0958-1669(93)90072-5

[4] HUMAN MONOCLONAL FAB FRAGMENTS DERIVED FROM A COMBINATORIAL LIBRARY BIND TO RESPIRATORY SYNCYTIAL VIRUS-F GLYCOPROTEIN AND NEUTRALIZE INFECTIVITY [J].

BARBAS, CF ;

CROWE, JE ;

CABABA, D ;

JONES, TM ;

ZEBEDEE, SL ;

MURPHY, BR ;

CHANOCK, RM ;

BURTON, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10164-10168

[5] ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE [J].

BARBAS, CF ;

KANG, AS ;

LERNER, RA ;

BENKOVIC, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :7978-7982

[6] SOMATIC DIVERSIFICATION OF IMMUNOGLOBULIN HEAVY-CHAIN VDJ GENES - EVIDENCE FOR SOMATIC GENE CONVERSION IN RABBITS [J].

BECKER, RS ;

KNIGHT, KL .

CELL, 1990, 63 (05) :987-997

[7] RECOMBINANT HUMAN FAB TO GLYCOPROTEIN-D NEUTRALIZES INFECTIVITY AND PREVENTS CELL-TO-CELL TRANSMISSION OF HERPES-SIMPLEX VIRUS-1 AND VIRUS-2 IN-VITRO [J].

BURIONI, R ;

WILLIAMSON, RA ;

SANNA, PP ;

BLOOM, FE ;

BURTON, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :355-359

[8] A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS [J].

BURTON, DR ;

BARBAS, CF ;

PERSSON, MAA ;

KOENIG, S ;

CHANOCK, RM ;

LERNER, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10134-10137

[9] HIGH-LEVEL ESCHERICHIA-COLI EXPRESSION AND PRODUCTION OF A BIVALENT HUMANIZED ANTIBODY FRAGMENT [J].

CARTER, P ;

KELLEY, RF ;

RODRIGUES, ML ;

SNEDECOR, B ;

COVARRUBIAS, M ;

VELLIGAN, MD ;

WONG, WLT ;

ROWLAND, AM ;

KOTTS, CE ;

CARVER, ME ;

YANG, M ;

BOURELL, JH ;

SHEPARD, HM ;

HENNER, D .

BIO-TECHNOLOGY, 1992, 10 (02) :163-167

[10]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

← 1 2 3 4 5 →