SYNTHESIS OF DABSYLHYDRAZINE AND ITS USE IN THE CHROMATOGRAPHIC DETERMINATION OF MONOSACCHARIDES BY THIN-LAYER AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：35

作者：

LIN, JK

WU, SS

机构：

[1] Natl Taiwan Univ, Taipei, Taiwan, Natl Taiwan Univ, Taipei, Taiwan

来源：

ANALYTICAL CHEMISTRY | 1987年 / 59卷 / 09期

关键词：

BIOCHEMISTRY - Glucose in Body Fluids - CARBOHYDRATES - Chromatographic Analysis;

D O I：

10.1021/ac00136a014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The chromophoric reagent dabsylhydrazine is synthesized from the reaction of dabsyl chloride with hydrazine. The application of this new reagent in the thin-layer chromatographic and high-performance liquid chromatographic analysis of monosaccharides is described. By use of a Nova-PAK C//1//8 reverse-phase column and a concave gradient system of water and acetonitrile as eluant, detection limits in the range of 10 pmol (2 ng) have been reached. The optimum pH for the formation of monosaccharide dabsylhydrazones is around 2-3.

引用

页码：1320 / 1326

页数：7

共 23 条

[1] A RAPID AND SENSITIVE METHOD FOR THE DETERMINATION OF MONOSACCHARIDES AS THEIR DANSYL HYDRAZONES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].