THE ROLE OF RNA-POLYMERASE IN TRANSCRIPTIONAL FIDELITY

被引：34

作者：

LIBBY, RT ^{[1
]}

GALLANT, JA ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT GENET,SK-50,SEATTLE,WA 98195

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb01872.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The overall transcription of DNA has previously been demonstrated to proceed at extremely high levels of accuracy. We review the evidence that the process of transcription is subject to proof-reading in the Hopfield sense. In addition, we speculate that the proof-reading activity associated with transcription is subject to cyclical phase transitions. That is, during periods of low processivity associated with initiation, RNA synthesis is relatively imprecise. The transition to the elongation phase of RNA synthesis, characterized by a shift to high processivity, is accompanied by enhanced proof-reading. A model for the damping of transcriptional errors, based on a PPi-mediated processive pyrophosphorolysis reaction, is discussed in terms of pausing during transcription.

引用

页码：999 / 1004

页数：6

共 35 条

[11] THE ENERGY RELAY - A PROOFREADING SCHEME BASED ON DYNAMIC COOPERATIVITY AND LACKING ALL CHARACTERISTIC SYMPTOMS OF KINETIC PROOFREADING IN DNA-REPLICATION AND PROTEIN-SYNTHESIS [J].