A RAPID AND NONINVASIVE SELECTION OF TRANSGENIC EMBRYOS BEFORE IMPLANTATION USING GREEN FLUORESCENT PROTEIN (GFP)

被引：129

作者：

IKAWA, M

KOMINAMI, K

YOSHIMURA, Y

TANAKA, K

NISHIMUNE, Y

OKABE, M

机构：

[1] OSAKA UNIV, MICROBIAL DIS RES INST, SUITA, OSAKA 565, JAPAN

[2] OSAKA UNIV, FAC PHARMACEUT SCI, SUITA, OSAKA 565, JAPAN

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

关键词：

GREEN FLUORESCENT PROTEIN; TRANSGENIC MOUSE; PREIMPLANTATION EMBRYO; BETA-ACTIN PROMOTER; PCR; MARKER;

D O I：

10.1016/0014-5793(95)01162-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos, The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a fluorescent microscope, All the green embryos were shown to carry the transgene by PCR analysis, Taking advantage of the detection of GFP expression can be done non-invasively, the selected embryos mere demonstrated to be able to developed to term with 100% of accuracy of the selection.

引用

页码：125 / 128

页数：4

共 23 条

[1]

BONNEROT C, 1993, METHOD ENZYMOL, V225, P451

[2] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].