COMPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSES OF CHOLESTEROL AND ITS OXIDATION-PRODUCTS USING DIODE-ARRAY ULTRAVIOLET AND LASER LIGHT-SCATTERING DETECTION

被引：20

作者：

KERMASHA, S ^{[1
]}

KUBOW, S ^{[1
]}

GOETGHEBEUR, M ^{[1
]}

机构：

[1] MCGILL UNIV,SCH DIETET & HUMAN NUTR,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 685卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)00718-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative high-performance liquid chromatographic (HPLC) analyses of cholesterol and its oxidization products (COPS) showed that cholesterol, cholestane, 7-ketocholesterol and 7 alpha, 7 beta-, 20- and 25-hydroxycholesterol were detected by both ultraviolet (UV) and laser light-scattering detection (LLSD). In addition, the use of LLSD allowed the detection of cholestanetriol and alpha- and beta-epoxides. The limits of detection of COPS varied from 0.5 to 25 mu g/ml, depending on both the compound and the detector. The HPLC analyses demonstrated a linear correlation between the UV response and concentrations of products in the range 0-500 mu g/ml whereas a linear correlationship with LLSD was obtained by plotting logarithmic coordinates in the same range.

引用

页码：229 / 235

页数：7

共 27 条

[1] CAPILLARY GC QUANTIFICATION OF CHOLESTEROL OXIDATION-PRODUCTS IN PLASMA-LIPOPROTEINS OF FASTED HUMANS [J].

ADDIS, PB ;

EMANUEL, HA ;

BERGMANN, SD ;

ZAVORAL, JH .

FREE RADICAL BIOLOGY AND MEDICINE, 1989, 7 (02) :179-182

[2] OCCURRENCE OF LIPID OXIDATION-PRODUCTS IN FOODS [J].

ADDIS, PB .

FOOD AND CHEMICAL TOXICOLOGY, 1986, 24 (10-11) :1021-1030

[3]

ADDIS PB, 1989, FOOD TOXICOLOGY PERS, pCH11

[4]

CHEILLAN F, 1989, LIPIDS, V24, P225

[5] HPLC METHOD FOR QUANTITATION OF CHOLESTEROL AND 4 OF ITS MAJOR OXIDATION-PRODUCTS IN MUSCLE AND LIVER-TISSUES [J].

CSALLANY, AS ;

KINDOM, SE ;

ADDIS, PB ;

LEE, JH .

LIPIDS, 1989, 24 (07) :645-651

[6] TRACE ENRICHMENT AND SEPARATION OF CHOLESTEROL OXIDATION-PRODUCTS BY ADSORPTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

CSIKY, I .

JOURNAL OF CHROMATOGRAPHY, 1982, 241 (02) :381-389

[7] ISOLATION AND IDENTIFICATION OF CHOLESTEROL ALPHA-OXIDE AND OTHER MINOR STEROLS IN HUMAN SERUM [J].

GRAY, MF ;

LAWRIE, TDV ;

BROOKS, CJW .

LIPIDS, 1971, 6 (11) :836-&

[8]

HEFTMANN E, 1979, J CHROMATOGR, V165, P238

[9]

JAVITT NB, 1981, J BIOL CHEM, V256, P2644

[10] DETERMINATION OF THE POSITIONAL DISTRIBUTION OF FATTY-ACIDS IN BUTTERFAT TRIACYLGLYCEROLS [J].

KERMASHA, S ;

KUBOW, S ;

SAFARI, M ;

REID, A .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1993, 70 (02) :169-173

← 1 2 3 →