METHANOL AND METHYLAMINE UTILIZATION RESULT FROM MUTATIONAL EVENTS IN THIOSPHAERA-PANTOTROPHA

With choline as carbon source Thiosphaera pantotropha GB17 grew with a doubling time (t(d)) of 6 h. The cellular yield was 55.8 g dry cell weight per mol of choline, indicating that its methyl moieties were used for growth. However, T. pantotropha was unable to grow with methanol or with methylamine as carbon source. Mutants were isolated from liquid or from solid media able to grow with methanol (Mox+) as carbon or methylamine as nitrogen source (Mam+). The Mox+ mutant GB17M grew with a mean t(d) of 11.7 h and a growth yield of 8.9 g dry cell weight per mol of methanol. Diauxic growth of strain GB17M was observed with mixtures of pyruvate and methanol as substrates in batch culture. Methanol led to the formation of methanol dehydrogenase, formate dehydrogenase, ribulosebisphosphate carboxylase and of a soluble cytochrome c-551.5. Tn5-insertional mutants defective in the thiosulfate oxidizing enzyme system or in hydrogenase acquired the Mox+ phenotype. However, Tn5-insertional mutants defective in either a c-type cytochrome or the molybdenum cofactor did not mutate to the Mox+ phenotype, indicating common functions in thiosulfate and in methanol metabolism.