POLYGLUTARALDEHYDE - NEW REAGENT FOR COUPLING PROTEINS TO MICROSPHERES AND FOR LABELING CELL-SURFACE RECEPTORS .2. SIMPLIFIED LABELING METHOD BY MEANS OF NON-MAGNETIC AND MAGNETIC POLYGLUTARALDEHYDE MICROSPHERES

被引：62

作者：

MARGEL, S

ZISBLATT, S

REMBAUM, A

机构：

[1] Jet Propulsion Laboratory, California Institute of Technology, Pasadena

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 28卷 / 3-4期

关键词：

D O I：

10.1016/0022-1759(79)90199-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Procedures were developed for the synthesis of a new immunoreagent in form of polyglutaraldehyde (PGL) microspheres in sizes ranging from about 50 nm to 1.5 μm. Addition of fluorochromes during synthesis yielded microspheres of high fluorescence intensity. By carrying out the polymerization of glutaraldehyde in presence of iron oxide, magnetic PGL microspheres were produced. Antibody conjugates obtained by interaction of PGL microspheres with immunoglobulins were used to label human red blood cells (RBC) and lymphocytes. A simple method for the separation of magnetically labeled human RBC from unlabeled cells was demonstrated. © 1979.

引用

页码：341 / 353

页数：13

共 25 条

[11]

LYNN N, 1976, CLIN CHIM ACTA, V69, P387

[12] NEW IMMUNOLATEX SPHERES - VISUAL MARKERS OF ANTIGENS ON LYMPHOCYTES FOR SCANNING ELECTRON-MICROSCOPY [J].