SPECIFIC HYBRIDIZATION PROBES FOR MOUSE ALPHA-2(IX) AND ALPHA-1(X) COLLAGEN MESSENGER-RNAS

被引：30

作者：

ELIMA, K

METSARANTA, M

KALLIO, J

PERALA, M

EEROLA, I

GAROFALO, S

DECROMBRUGGHE, B

VUORIO, E

机构：

[1] UNIV TURKU,DEPT MED BIOCHEM,KIINAMYLLYNKATU 10,SF-20520 TURKU 52,FINLAND

[2] UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT MOLEC GENET,HOUSTON,TX 77030

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1130卷 / 01期

关键词：

CARTILAGE; COLLAGEN; GENE EXPRESSION; MESSENGER RNA; RECOMBINANT DNA; (MOUSE);

D O I：

10.1016/0167-4781(92)90465-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used polymerase chain reaction (PCR) technology and available cross-species sequence information to construct cDNA probes for mouse alpha-2(IX) and alpha-1(X) collagen transcripts. Sequencing confirmed the identification of the clones. Northern analysis proved sufficient divergence of the cloned sequences from other collagen transcripts: specific detection of the mouse 2.9 kb alpha-2(IX) and 3.3 kb alpha-1(X) collagen mRNAs was seen under normal hybridization and washing conditions.

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页码：78 / 80

页数：3

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