OPTIMIZATION OF PH FOR THE SEPARATION OF ORGANIC-ACIDS IN CAPILLARY ZONE ELECTROPHORESIS

被引：107

作者：

SMITH, SC ^{[1
]}

KHALEDI, MG ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,DEPT CHEM,BOX 8204,RALEIGH,NC 27695

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 03期

关键词：

D O I：

10.1021/ac00051a001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The migration behavior and optimum separation of a set of substituted phenols is presented. A mathematical model that describes mobility in CZE in terms of fundamental constants of each solute (acid dissociation constant K(a), mobility of the dissociated acid mu(A)-) and the pH of the buffer was used to predict the mobility of each solute as a function of pH. The resolution between each peak pair was calculated, and a window diagram of minimum resolution as a function of pH was employed in determining the optimum pH. A comparison of the predicted and actual electropherograms shows the usefulness of this technique.

引用

页码：193 / 198

页数：6

共 27 条

[1] Ahuja S., 1989, SELECTIVITY DETECTAB
[2] HIGH-PERFORMANCE CAPILLARY ELECTROPHORETIC SEPARATION OF BASES, NUCLEOSIDES, AND OLIGONUCLEOTIDES - RETENTION MANIPULATION VIA MICELLAR SOLUTIONS AND METAL ADDITIVES
COHEN, AS
TERABE, S
SMITH, JA
KARGER, BL
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (07) : 1021 - 1027
[3] SEPARATION OF OPTICAL ISOMERS BY CAPILLARY ZONE ELECTROPHORESIS BASED ON HOST GUEST COMPLEXATION WITH CYCLODEXTRINS
FANALI, S
[J]. JOURNAL OF CHROMATOGRAPHY, 1989, 474 (02): : 441 - 446
[4] FUJIWARA S, 1988, J CHROMATOGR, V447, P133, DOI 10.1016/S0021-9673(01)91462-1
[5] GENERATION OF VARIANCE, THEORETICAL PLATES, RESOLUTION, AND PEAK CAPACITY IN ELECTROPHORESIS AND SEDIMENTATION
GIDDINGS, JC
[J]. SEPARATION SCIENCE, 1969, 4 (03): : 181 - &
[6] ELECTROKINETIC RESOLUTION OF AMINO-ACID ENANTIOMERS WITH COPPER(II) ASPARTAME SUPPORT ELECTROLYTE
GOZEL, P
GASSMANN, E
MICHELSEN, H
ZARE, RN
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (01) : 44 - 49
[7] USE OF COMPLEXING AGENTS FOR SELECTIVE SEPARATION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS - CHIRAL RESOLUTION VIA CYCLODEXTRINS INCORPORATED WITHIN POLYACRYLAMIDE-GEL COLUMNS
GUTTMAN, A
PAULUS, A
COHEN, AS
GRINBERG, N
KARGER, BL
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 448 (01): : 41 - 53
[8] TABLE OF ISOTACHOPHORETIC INDEXES .1. SIMULATED QUALITATIVE AND QUANTITATIVE INDEXES OF 287 ANIONIC SUBSTANCES IN THE RANGE PH 3-10
HIROKAWA, T
NISHINO, M
AOKI, N
KISO, Y
SAWAMOTO, Y
YAGI, T
AKIYAMA, J
[J]. JOURNAL OF CHROMATOGRAPHY, 1983, 271 (02): : D1 - D106
[9] USE OF CYCLODEXTRINS IN ISOTACHOPHORESIS .3. PURITY CONTROL OF NAFTIDROFURYL HYDROGENOXALATE AND SOME OF ITS SYNTHESIS INTERMEDIATES
JELINEK, I
DOHNAL, J
SNOPEK, J
SMOLKOVAKEULEMANSOVA, E
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 435 (03): : 496 - 500
[10] CAPILLARY ZONE ELECTROPHORESIS
JORGENSON, JW
LUKACS, KD
[J]. SCIENCE, 1983, 222 (4621) : 266 - 272

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