3-DIMENSIONAL CRYSTAL-STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST-33258

被引：121

作者：

VEGA, MC

SAEZ, IG

AYMAMI, J

ERITJA, R

VANDERMAREL, GA

VANBOOM, JH

RICH, A

COLL, M

机构：

[1] CSIC, CTR INVEST & DESENVOLUPAMENT, DEPT BIOL MOLEC & CELLULAR, E-08034 BARCELONA, SPAIN

[2] UNIV POLITECN CATALUNYA, DEPT ENGN QUIM, BARCELONA, SPAIN

[3] LEIDEN STATE UNIV, GORLAEUS LABS, 2300 RA LEIDEN, NETHERLANDS

[4] MIT, DEPT BIOL, CAMBRIDGE, MA USA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 222卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1994.tb18917.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular structure of the DNA A-tract dodecamer d(CGCAAATTTGCG) complexed with the drug Hoechst 33258 has been determined by X-ray diffraction analysis. The Hoechst molecule binds in the DNA minor groove covering the sequence AATTT of the central A-tract, with the piperazine group close to one of the GC regions. The drug molecule makes two three-centered hydrogen bonds from the nitrogen atoms of the benzimidazole rings to the N3 and O2 atoms of the DNA bases. Although a high propeller twist is observed in the A-tract, only one unsymmetrical three-centered hydrogen bond is present in the DNA major groove. The structure is compared with other minor-groove-binding drug complexes and the influence of these drugs on DNA A-tracts is discussed.

引用

页码：721 / 726

页数：6

共 32 条

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