SEPARATION OF 24 DANSYLAMINO ACIDS BY CAPILLARY ELECTROPHORESIS WITH A NONIONIC SURFACTANT

被引：49

作者：

MATSUBARA, N

TERABE, S

机构：

[1] Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 680卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80081-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of 24 dansylamino acids was investigated by capillary electrophoresis with an additive of micelles of a non-ionic surfactant, Tween 20. Although two pairs of peaks, norvaline and methionine derivatives, and didansyltyrosine and solvent (methanol), did not show good resolution, other dansylamino acids were well separated within 70 min using 100 mM Tween 20 and pH 2.40. The theoretical plate numbers calculated for dansylamino acids were 28000-111000 with a 19-cm capillary column.

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页码：311 / 315

页数：5

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