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THE NA+/H+ EXCHANGER IS PHOSPHORYLATED IN HUMAN PLATELETS IN RESPONSE TO ACTIVATING AGENTS

被引：48
作者：
LIVNE, AA
SARDET, C
POUYSSEGUR, J
机构：
[1] UNIV NICE,CNRS,CTR BIOCHIM,F-06034 NICE,FRANCE
[2] BEN GURION UNIV NEGEV,DEPT LIFE SCI,IL-84105 BEER SHEVA,ISRAEL
来源：
FEBS LETTERS | 1991年 / 284卷 / 02期
关键词：
PLATELET; THROMBIN; NA+/H+ EXCHANGER; PHOSPHORYLATION; OKADAIC ACID;
D O I：
10.1016/0014-5793(91)80689-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alpha-Thrombin, phorbol esters (PMA) and 1,2-diacylglycerol (DAG), three activators of the amiloride-sensitive Na+/H+ exchange in human platelets, rapidly increase the intracellular pH and the level of phosphorylation of the Na+/H+ exchanger protein (NHE1). This stimulatory effect is suppressed by staurosporine, a potent kinase inhibitor, and increased by okadaic acid, a potent inhibitor of phosphatase 1 and 2A. The modulations of NHE1 phosphorylation by these factors correlate well with their effects on platelet pH. Thus, we conclude that in platelets (i) Na+/H+ exchange is mediated by NHE1, and (ii) platelet activating agents stimulate NHE1 via the modulation of the kinase/phosphatase equilibrium.
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页码：219 / 222
页数：4
相关论文
共 23 条
[11]   A SPECIFIC MUTATION ABOLISHING NA+/H+ ANTIPORT ACTIVITY IN HAMSTER FIBROBLASTS PRECLUDES GROWTH AT NEUTRAL AND ACIDIC PH [J].
POUYSSEGUR, J ;
SARDET, C ;
FRANCHI, A ;
LALLEMAIN, G ;
PARIS, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (15) :4833-4837
[12]  
SARDER C, IN PRESS NATO ASI SE
[13]   GROWTH-FACTORS INDUCE PHOSPHORYLATION OF THE NA+/H+ ANTIPORTER, A GLYCOPROTEIN OF 110-KD [J].
SARDET, C ;
COUNILLON, L ;
FRANCHI, A ;
POUYSSEGUR, J .
SCIENCE, 1990, 247 (4943) :723-726
[14]   MOLECULAR-CLONING, PRIMARY STRUCTURE, AND EXPRESSION OF THE HUMAN GROWTH FACTOR-ACTIVATABLE NA+/H+ ANTIPORTER [J].
SARDET, C ;
FRANCHI, A ;
POUYSSEGUR, J .
CELL, 1989, 56 (02) :271-280
[15]   THROMBIN STIMULATES NA+-H+ EXCHANGE ACROSS THE HUMAN-PLATELET PLASMA-MEMBRANE [J].
SIFFERT, W ;
FOX, G ;
MUCKENHOFF, K ;
SCHEID, P .
FEBS LETTERS, 1984, 172 (02) :272-274
[16]  
SIFFERT W, 1988, J BIOL CHEM, V263, P4223
[17]   A PHORBOL ESTER AND 1-OLEOYL-2-ACETYLGLYCEROL INDUCE NA+/H+ EXCHANGE IN HUMAN-PLATELETS [J].
SIFFERT, W ;
SCHEID, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 141 (01) :13-19
[18]   NA+/H+ EXCHANGE AS A MODULATOR OF PLATELET ACTIVATION [J].
SIFFERT, W ;
AKKERMAN, JWN .
TRENDS IN BIOCHEMICAL SCIENCES, 1988, 13 (04) :148-151
[19]  
SWEATT JD, 1986, J BIOL CHEM, V261, P8660
[20]   MOLECULAR-CLONING AND EXPRESSION OF THE MAJOR PROTEIN KINASE-C SUBSTRATE OF PLATELETS [J].
TYERS, M ;
RACHUBINSKI, RA ;
STEWART, MI ;
VARRICHIO, AM ;
SHORR, RGL ;
HASLAM, RJ ;
HARLEY, CB .
NATURE, 1988, 333 (6172) :470-473
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