LIPOPOLYSACCHARIDE PROMOTED OPENING OF THE PORIN CHANNEL

被引：27

作者：

ISHII, J ^{[1
]}

NAKAE, T ^{[1
]}

机构：

[1] TOKAI UNIV,SCH MED,DEPT MOLEC LIFE SCI,143 SHIMOKASUYA,ISEHARA,KANAGAWA 25911,JAPAN

来源：

FEBS LETTERS | 1993年 / 320卷 / 03期

关键词：

CHANNEL; GATE; PORIN; CONDUCTIVITY; ANTIBIOTIC; LIPOPOLYSACCHARIDE;

D O I：

10.1016/0014-5793(93)80597-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show here that the imipenem (a carbapenem, beta-lactam antibiotic)-permeable porin channels (protein D2 or OprD2) of Pseudomonas aeruginosa were closed mostly in the lipopolysaccharide (LPS)-free membrane and were openable by adding LPS to the membrane as assayed by ion conductivity measurements using planar lipid bilayers. Open and closed states of the OprD2 channels exhibited conductivities of about 400 and 30 pS, respectively, in 1 M NaCl. The OprD2 channel in the LPS-containing membrane showed very rapid opening and closing events in a second order and the duration of closure became longer at low membrane potentials.

引用

页码：251 / 255

页数：5

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