PREPARATIVE SEPARATION AND ISOLATION OF 3 ALPHA-BITTER ACIDS FROM HOP, HUMULUS-LUPULUS L, BY CENTRIFUGAL PARTITION CHROMATOGRAPHY

被引：21

作者：

HERMANSLOKKERBOL, ACJ

VERPOORTE, R

机构：

[1] Leiden/Amsterdam Center for Drug Research, Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, 2300 RA Leiden

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 664卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80627-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Centrifugal partition chromatography was used for the preparative separation of bitter acids from a crude supercritical carbon dioxide extract of hop cones. The main alpha-acids, humulone, cohumulone and adhumulone were obtained pure in one chromatographic run with the system toluene-0.1 M triethanolamine . HCl pH 8.4 in water. The two-phase system was optimized for pH and the effect of ethylene glycol on the separation was investigated.

引用

页码：45 / 53

页数：9

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