FORMATION OF [LEU5]ENKEPHALIN FROM DYNORPHIN-A(1-8) BY RAT CENTRAL NERVOUS-TISSUE INVITRO

被引：26

作者：

DIXON, DM ^{[1
]}

TRAYNOR, JR ^{[1
]}

机构：

[1] LOUGHBOROUGH UNIV TECHNOL,DEPT CHEM,LOUGHBOROUGH LE11 3TU,LEICS,ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 04期

关键词：

Dynorphin A(1–8); Endopeptidase; Metabolism; Peptidase inhibitors; Rat brain and spinal cord; Leu[!sup]5[!/sup]]Enkephalin;

D O I：

10.1111/j.1471-4159.1990.tb01972.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[3H]Dynorphin A(1–8) is readily metabolised by rat lumbosacral spinal cord tissue in vitro, affording a variety of products including a significant amount (20% recovered activity) of [3H][Leu5]enkephalin. In the presence of the peptidase inhibitors bestatin, captopril, thiorphan, and leucylleucine, [3H][Leu5]enkephalin was the major metabolic product, accounting for 60% of recovered activity. Production of [3H][Leu5]enkephalin was seen across all gross brain regions. The enzyme responsible for the cleavage has an optimal substrate length of 8–13 amino acids and is inhibited b N‐[1‐(RS)‐carboxy‐2‐phenylethyl]‐Ala‐Ala‐Phe‐p‐aminobenzoate, a site‐directed inhibitor of the metalloendopeptidase EC 3.4.24.15. However the enzymic breakdown also has properties in common with involvement of endo‐oligopeptidase A. Possible consequences of the formation of [Leu5]enkephalin from the smaller dynorphins are discussed. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1379 / 1385

页数：7

共 28 条

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