INDUCTION OF HUMAN TISSUE-PLASMINOGEN ACTIVATOR IN THE MAMMARY-GLAND OF TRANSGENIC GOATS

被引：73

作者：

EBERT, KM ^{[1
]}

DITULLIO, P ^{[1
]}

BARRY, CA ^{[1
]}

SCHINDLER, JE ^{[1
]}

AYRES, SL ^{[1
]}

SMITH, TE ^{[1
]}

PELLERIN, LJ ^{[1
]}

MEADE, HM ^{[1
]}

DENMAN, J ^{[1
]}

ROBERTS, B ^{[1
]}

机构：

[1] GENZYME TRANSGEN CORP,FRAMINGHAM,MA 01701

来源：

BIO-TECHNOLOGY | 1994年 / 12卷 / 07期

关键词：

D O I：

10.1038/nbt0794-699

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three transgenic females from a first generation transgenic male were induced to lactate between Il and 12 months of age using a series of estrogen and progesterone injections. The milk contained human longer acting tissue plasminogen activator (LAtPA) at comparable concentrations (1-3 mg/ml) as occurred in the original founder female, In addition, the transgenic male was induced with a hormonal regime and was shown to produce 0.85 mg/ml of LAtPA. Milk protein gels indicated that the milk products (casein, IgG) were essentially normal. These experiments show that expression data for this vector can be evaluated in a shorter period of time in dairy goats than would be required through normal gestation and lactation schedules and can be used to identify the relative expression of transgenes in mammary tissue that would occur during normal lactation.

引用

页码：699 / 702

页数：4

共 14 条

[1] INDUCED LACTATION IN DAIRY EWES BY VARIOUS BRIEF HORMONE TREATMENTS [J].