IN-VITRO ACTIVITY OF PARAHERQUAMIDE AGAINST THE FREE-LIVING STAGES OF HAEMONCHUS-CONTORTUS, TRICHOSTRONGYLUS-COLUBRIFORMIS AND OSTERTAGIA-CIRCUMCINCTA

Paraherquamide, an oxindole alkaloid recently reported to have potent nematocidal activity, was shown to have a marked inhibitory effect on the motility of the free-living larval stages of H. contortus, T. colubriformis and O. circumcincta. The effect of paraherquamide on larval motility could be distinguished from that caused by levamisole and the avermectins. After 72 h exposure, the concentration of paraherquamide required to inhibit the motility of 50% of L3 larvae present was 0.033, 0.058 and 2.7 mug ml-1 for O. circumcincta, T. colubriformis and H. contortus, respectively. Ivermectin (IVM)-resistant isolates of H. contortus were significantly more sensitive to the paralytic effects of paraherquamide than IVM-susceptible isolates of this species. Paraherquamide had no effect on the time for development from the egg to the L3 larval stage of H. contortus, T. colubriformis and O. circumcincta.