CHARACTERIZATION OF A PROLATE-HEADED BACTERIOPHAGE OF LACTOBACILLUS-DELBRUECKII SUBSP LACTIS, AND ITS DNA HOMOLOGY WITH ISOMETRIC-HEADED PHAGES

被引：23

作者：

FORSMAN, P

机构：

[1] Department of Genetics, University of Oulu, Oulu

来源：

ARCHIVES OF VIROLOGY | 1993年 / 132卷 / 3-4期

关键词：

D O I：

10.1007/BF01309542

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new Lactobacillus delbrueckii subsp. lactis bacteriophage, JCL 1032, was characterized. JCL 1032 had a small, elongated prolate head, and a long non-contractile tail with cross-bars. The restriction map of JCL 1032 genome was constructed with five endonucleases. The genome was 45.8 kb in size, and it had cohesive ends (cos). Molecular masses of the phage structural proteins were also determined. JCL 1032 showed DNA homology with morphologically dissimilar, isometric-headed phages of Lb. delbrueckii (subsp. lactis and subsp. bulgaricus) when analyzed by Southern hybridization. Although in general JCL 1032 was only distantly related to isometric-headed phages, there were also a few short highly homologous (minimal homology 84%) DNA regions.

引用

页码：321 / 330

页数：10

共 23 条

[1] MORPHOLOGY OF BACTERIOPHAGES OF LACTOBACILLUS-BULGARICUS, LACTOBACILLUS-LACTIS AND LACTOBACILLUS-HELVETICUS [J].