DISTRIBUTION AND PARTIAL CHARACTERIZATION OF IMMUNOREACTIVITY TO THE PUTATIVE C-TERMINUS OF RAT PANCREASTATIN

被引：48

作者：

CURRY, WJ ^{[1
]}

JOHNSTON, CF ^{[1
]}

SHAW, C ^{[1
]}

BUCHANAN, KD ^{[1
]}

机构：

[1] QUEENS UNIV BELFAST,INST CLIN SCI,DEPT MED,WELLCOME RES LABS,GROSVENOR RD,BELFAST BT12 6BJ,ANTRIM,NORTH IRELAND

来源：

REGULATORY PEPTIDES | 1990年 / 30卷 / 03期

基金：

英国医学研究理事会;

关键词：

Chromogranin A; Gel permeation chromatography; HPLC; Immunocytochemistry; Pancreastatin; Radioimmunoassay;

D O I：

10.1016/0167-0115(90)90096-F

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The presumptive C-terminal nonapeptide of rat pancreastatin was synthesised based upon the sequence of rat chromogranin A (CGA) analogous to that of porcine pancreastatin as contained within porcine CGA. Antisera were produced which were used to determine the qualitative and quantitative distribution of pancreastatin-like immunoreactivity in rat tissues by immunocytochemistry and radioimmunoassay respectively. Pancreastatin-like immunoreactivity was most abundant in pituitary, adrenal, gastric corpus and thyroid with considerably lower levels detected in the remainder of the gastroentero-pancreatic system and brain. Immunoreactivity was localised exclusively in endocrine cells and the relative abundance of immunoreactive cells paralleled the levels obtained radioimmunometrically. Chromatographic characterisation of pancreastatin-like immunoreactivity revealed molecular heterogeneity. Immunoreactive peptides of similar size to synthetic rat pancreastatin were present in gastrointestinal tissues and thyroid. These data indicate a tissue specific processing of CGA in the rat. © 1990.

引用

页码：207 / 219

页数：13

共 29 条

[1] THE PRIMARY STRUCTURE OF BOVINE CHROMOGRANIN-A - A REPRESENTATIVE OF A CLASS OF ACIDIC SECRETORY PROTEINS COMMON TO A VARIETY OF PEPTIDERGIC CELLS
BENEDUM, UM
BAEUERLE, PA
KONECKI, DS
FRANK, R
POWELL, J
MALLET, J
HUTTNER, WB
[J]. EMBO JOURNAL, 1986, 5 (07) : 1495 - 1502
[2] LABELING OF PROTEINS TO HIGH SPECIFIC RADIOACTIVITIES BY CONJUGATION TO A I-125-CONTAINING ACYLATING AGENT - APPLICATION TO RADIOIMMUNOASSAY
BOLTON, AE
HUNTER, WM
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (03) : 529 - 538
[3] PANCREASTATIN DISTRIBUTION AND PLASMA-LEVELS IN THE PIG
BRETHERTONWATT, D
GHATEI, MA
BISHOP, AE
FACER, P
FAHEY, M
HEDGES, M
WILLIAMS, G
VALENTINO, KL
TATEMOTO, K
ROTH, K
POLAK, JM
BLOOM, SR
[J]. PEPTIDES, 1988, 9 (05) : 1005 - 1014
[4] BUFFA R, 1988, BASIC APPL HISTOCHEM, V32, P471
[5] SELECTIVE LOCALIZATION OF THE PARATHYROID SECRETORY PROTEIN-I ADRENAL-MEDULLA CHROMOGRANIN-A PROTEIN FAMILY IN A WIDE VARIETY OF ENDOCRINE-CELLS OF THE RAT
COHN, DV
ELTING, JJ
FRICK, M
ELDE, R
[J]. ENDOCRINOLOGY, 1984, 114 (06) : 1963 - 1974
[6] PANCREASTATIN AND ISLET HORMONE-RELEASE
EFENDIC, S
TATEMOTO, K
MUTT, V
QUAN, C
CHANG, D
OSTENSON, CG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) : 7257 - 7260
[7] PEPTIDE FUNCTION - IS CHROMOGRANIN A PROHORMONE
EIDEN, LE
[J]. NATURE, 1987, 325 (6102) : 301 - 301
[8] CELL-FREE AND CELLULAR SYNTHESIS OF CHROMOGRANIN-A AND CHROMOGRANIN-B OF BOVINE ADRENAL-MEDULLA
FALKENSAMMER, G
FISCHERCOLBRIE, R
RICHTER, K
WINKLER, H
[J]. NEUROSCIENCE, 1985, 14 (02) : 735 - 746
[9] IMMUNOREACTIVITIES OF GASTRIN (G-) CELLS .2. NONSPECIFIC-BINDING OF IMMUNOGLOBULINS TO G-CELLS BY IONIC INTERACTIONS
GRUBE, D
[J]. HISTOCHEMISTRY, 1980, 66 (02) : 149 - 167
[10] CHROMOGRANIN-A (CGA) IN THE GASTROENTEROPANCREATIC (GEP) ENDOCRINE SYSTEM .1. CGA IN THE MAMMALIAN ENDOCRINE PANCREAS
GRUBE, D
AUNIS, D
BADER, F
CETIN, Y
JORNS, A
YOSHIE, S
[J]. HISTOCHEMISTRY, 1986, 85 (06) : 441 - 452

← 1 2 3 →