DISTRIBUTION AND PARTIAL CHARACTERIZATION OF IMMUNOREACTIVITY TO THE PUTATIVE C-TERMINUS OF RAT PANCREASTATIN

被引：48

作者：

CURRY, WJ ^{[1
]}

JOHNSTON, CF ^{[1
]}

SHAW, C ^{[1
]}

BUCHANAN, KD ^{[1
]}

机构：

[1] QUEENS UNIV BELFAST,INST CLIN SCI,DEPT MED,WELLCOME RES LABS,GROSVENOR RD,BELFAST BT12 6BJ,ANTRIM,NORTH IRELAND

来源：

REGULATORY PEPTIDES | 1990年 / 30卷 / 03期

基金：

英国医学研究理事会;

关键词：

Chromogranin A; Gel permeation chromatography; HPLC; Immunocytochemistry; Pancreastatin; Radioimmunoassay;

D O I：

10.1016/0167-0115(90)90096-F

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The presumptive C-terminal nonapeptide of rat pancreastatin was synthesised based upon the sequence of rat chromogranin A (CGA) analogous to that of porcine pancreastatin as contained within porcine CGA. Antisera were produced which were used to determine the qualitative and quantitative distribution of pancreastatin-like immunoreactivity in rat tissues by immunocytochemistry and radioimmunoassay respectively. Pancreastatin-like immunoreactivity was most abundant in pituitary, adrenal, gastric corpus and thyroid with considerably lower levels detected in the remainder of the gastroentero-pancreatic system and brain. Immunoreactivity was localised exclusively in endocrine cells and the relative abundance of immunoreactive cells paralleled the levels obtained radioimmunometrically. Chromatographic characterisation of pancreastatin-like immunoreactivity revealed molecular heterogeneity. Immunoreactive peptides of similar size to synthetic rat pancreastatin were present in gastrointestinal tissues and thyroid. These data indicate a tissue specific processing of CGA in the rat. © 1990.

引用

页码：207 / 219

页数：13

共 29 条

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