MOLECULAR-CLONING OF G-PROTEINS AND PHOSPHODIESTERASES FROM RAT TASTE CELLS

To identify and characterize those proteins involved in taste transduction, we cloned G proteins and phosphodiesterases from rat taste tissue. Using degenerate primers corresponding to conserved regions of G protein alpha subunits, the polymerase chain reaction was used to amplify and clone eight distinct cDNAs: alpha(i-2), alpha(i-3), alpha(12), alpha(14), a(s), alpha t-rod, alpha-t-cone and alpha gustducin. alpha(i-3), alpha(14), alpha(s), and alpha(t-rod) are more highly expressed in taste tissue than in the surrounding nonsensory tissue. a gustducin is only expressed in taste cells. Rod transducin had previously been found only in the rod cells of the retina, where it converts light stimulation of rhodopsin into activation of cGMP phosphodiesterase. The primary sequence of a gustducin shows striking similarities to rod transducin in the receptor interaction domain and the phosphodiesterase activation site. We propose that gustducin and transducin regulate phosphodiesterase activity in taste cells and that this may promote bitter transduction and inhibit sweet transduction. Consistent with this proposal, we cloned two types of cAMP PDE from taste tissue: dnc-1 and PDE-3.