VARIANT OF THE PYROANTIMONATE TECHNIQUE SUITABLE FOR LOCALIZATION OF CALCIUM IN OVARIAN TISSUE

被引:38
作者
WEAKLEY, BS
机构
关键词
D O I
10.1177/27.6.88471
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Osmium-pyroantimonate solutions for the precipitation of cations are unsuitable for use with delicate mammalian oocytes. A variant of the pyroantimonate technique employing a mixture of pyroantimonate and glutaraldehyde has been found to give successful and repeatable results if a fixation time of 4 hr is used. Calcium-containing antimonate precipitates were localized principally in nuclei, smooth endoplasmic reticulum, Golgi apparatus, mitochondria, and cytoplasmic processes of both oocytes and follicle cells, and along the plasma membrane in small oocytes. Deposits were also concentrated around the periphery of lipid droplets in the follicle cells. The presence of calcium in the precipitates was confirmed by x-ray microprobe analysis.
引用
收藏
页码:1017 / 1028
页数:12
相关论文
共 44 条
[11]  
HOPWOOD D, 1967, J ANAT, V101, P83
[12]   EFFECTS OF DIFFERENT PREPARATIVE PROCEDURES ON ULTRASTRUCTURE OF HAMSTER OVARY .1. EFFECTS OF VARIOUS FIXATIVE SOLUTIONS ON OVARIAN OOCYTES AND THEIR GRANULOSA-CELLS [J].
IQBAL, SJ ;
WEAKLEY, BS .
HISTOCHEMISTRY, 1974, 38 (02) :95-122
[13]   DISTRIBUTION OF INORGANIC CATIONS IN MOUSE TESTIS - ELECTRON MICROSCOPE AND MICROPROBE ANALYSIS [J].
KIERSZENBAUM, AL ;
LIBANATI, CM ;
TANDLER, CJ .
JOURNAL OF CELL BIOLOGY, 1971, 48 (02) :314-+
[14]   CRITIQUE ON K-PYROANTIMONATE METHOD FOR SEMIQUANTITATIVE ESTIMATION OF CATIONS IN CONJUNCTION WITH ELECTRON-MICROSCOPY [J].
KLEIN, RL ;
THURESON.A ;
YEN, SS .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1972, 20 (01) :65-&
[15]   ELEKTRONENMIKROSKOPISCHE LOKALISATION VON NA+ UND CL- IN ZELLEN UND GEWEBEN [J].
KOMNICK, H .
PROTOPLASMA, 1962, 55 (02) :414-418
[16]   ELECTRON PROBE ANALYSIS OF CATIONIC SPECIES IN PYROANTIMONATE PRECIPITATES IN EPON-EMBEDDED TISSUE [J].
LANE, BP ;
MARTIN, E .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1969, 17 (02) :102-&
[17]   SUBCELLULAR LOCALIZATION OF CALCIUM ION IN MAMMALIAN MYOCARDIUM [J].
LEGATO, MJ ;
LANGER, GA .
JOURNAL OF CELL BIOLOGY, 1969, 41 (02) :401-&
[18]  
LEHNINGER AL, 1967, ADV ENZYMOL RAMB, V29, P259
[19]  
MEIS LD, 1970, J BIOL CHEM, V245, P1883
[20]   CALCIUM-PUMP ACTIVITY OF RENAL PLASMA-MEMBRANE AND RENAL MICROSOMES [J].
MOORE, L ;
FITZPATRICK, DF ;
CHEN, TS ;
LANDON, EJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 345 (03) :405-418