ELASTIN METABOLISM DURING RECOVERY FROM IMPAIRED CROSS-LINK FORMATION

被引:26
作者
TINKER, D
ROMEROCHAPMAN, N
REISER, K
HYDE, D
RUCKER, R
机构
[1] UNIV CALIF DAVIS, COLL AGR & ENVIRONM SCI, DEPT NUTR, DAVIS, CA 95616 USA
[2] UNIV CALIF DAVIS, SCH VET MED, DEPT ANAT, DAVIS, CA 95616 USA
[3] UNIV CALIF DAVIS, SCH MED, DIV PULM MED, DAVIS, CA 95616 USA
关键词
D O I
10.1016/0003-9861(90)90267-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Accelerated proteolysis of tropoelastin and elastin occurs in the arteries of chicks rendered nutritionally copper-deficient. The process results in part from decreased elastin crosslinking. Repletion of copper-deficient chicks with copper causes a deposition of elastin that is proteinase resistant. Resistance to proteolysis is conferred within 48 h of dietary copper repletion. Deposition of aorta elastin to near normal values occurs after 3-4 days in copper-repleted chicks. Moreover, elastolysis was enhanced when the content of dehydrolysinonorleucine in elastin was abnormally low. The chemical modification of lysyl residue in elastin by citroconylation, however, did not influence the rate of elastolysis. We have shown previously that tropoelastin messenger RNA activity and synthesis are not influenced by dietary copper deprivation (1986, Biochem. J.236, 17-23). Rather, as demonstrated herein, the decrease in elastin content in arteries of copper-deficient birds appears to be more the result of enhanced degradation. Restoration of normal crosslinking restores deposition and imparts resistance to elastolysis. Moreover, serum appears to be a good source of elastolytic proteinases when the elastin substrate is partially or abnormally crosslinked. © 1990.
引用
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页码:326 / 332
页数:7
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