GENERATION OF SPECIES-SPECIFIC DNA PROBES FOR LEISHMANIA-AETHIOPICA

被引:16
作者
LASKAY, T
KIESSLING, R
DEWIT, TFR
WIRTH, DF
机构
[1] STATE UNIV LEIDEN HOSP,DEPT IMMUNOHAEMATOL & BLOOD BANK,2333 AA LEIDEN,NETHERLANDS
[2] HARVARD UNIV,SCH PUBL HLTH,DEPT TROP PUBL HLTH,BOSTON,MA 02115
关键词
LEISHMANIA-AETHIOPICA; KINETOPLAST DNA; DNA PROBE; DNA HYBRIDIZATION;
D O I
10.1016/0166-6851(91)90014-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here the cloning of kinetoplast DNA (kDNA) sequences from Leishmania aethiopica in order to develop a specific and sensitive method for the identification of the parasite. Analysis of the cloned kDNA sequences showed different taxonomic specificities demonstrating sequence diversity within the kinetoplast DNA. Cloned whole minicircle hybridized with all Old World Leishmania species tested. Some cloned fragments of minicircle kDNA hybridized with Leishmania species causing cutaneous leishmaniasis in the Old World, but not with the viscerotropic species. Two L. aethiopica-specific clones were found. These clones hybridized with all L. aethiopica isolates tested, but did not react with other Leishmania species. The nucleotide sequence of the L. aethiopica-specific R3 clone is presented. Clones hybridizing with only some of the L. aethiopica isolates were also identified, although none of them showed specificity only for isolates causing localized (LCL) or diffuse (DCL) form of cutaneous leishmaniasis in Ethiopia.
引用
收藏
页码:279 / 286
页数:8
相关论文
共 16 条
[11]  
Peters W, 1987, LEISHMANIASES BIOL M, V1
[12]   KINETOPLAST DNA MINICIRCLES - REGIONS OF EXTENSIVE SEQUENCE DIVERGENCE [J].
ROGERS, WO ;
WIRTH, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :565-569
[13]   KINETOPLAST DNA, MITOCHONDRIAL-DNA WITH A DIFFERENCE [J].
STUART, K .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1983, 9 (02) :93-104
[14]  
Wirth D.F., 1985, P127
[15]   RAPID IDENTIFICATION OF LEISHMANIA SPECIES BY SPECIFIC HYBRIDIZATION OF KINETOPLAST DNA IN CUTANEOUS LESIONS [J].
WIRTH, DF ;
PRATT, DM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (22) :6999-7003
[16]   LEISHMANIASIS AND MALARIA - NEW TOOLS FOR EPIDEMIOLOGIC ANALYSIS [J].
WIRTH, DF ;
ROGERS, WO ;
BARKER, R ;
DOURADO, H ;
SUESEBANG, L ;
ALBUQUERQUE, B .
SCIENCE, 1986, 234 (4779) :975-979