DETERMINATION OF HYALURONIC-ACID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THE OLIGOSACCHARIDES DERIVED THEREFROM AS 1-(4-METHOXY)PHENYL-3-METHYL-5-PYRAZOLONE DERIVATIVES

被引：27

作者：

KAKEHI, K ^{[1
]}

UEDA, M ^{[1
]}

SUZUKI, S ^{[1
]}

HONDA, S ^{[1
]}

机构：

[1] KINKI UNIV,FAC PHARMACEUT SCI,3-4-1 KOWAKAE,OSAKA,OSAKA 577,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 630卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80449-I

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hyaluronic acid (HA) was digested with various kinds of depolymerizing enzymes and the products were analysed by high-performance liquid chromatography (HPLC) after derivatization with 1-(4-methoxy)phenyl-3-methyl-5-pyrazolone (PMPMP). As hyaluronate 4-glycanohydrolase (EC 3.2.1.35) from sheep testis showed a high efficiency for depolymerization, giving the tetra- and hexasaccharides abundantly, and is inexpensive, a method for the specific determination of HA was established, based on digestion by this enzyme followed by determination of the tetra- or hexasaccharide derived therefrom as the PMPMP derivatives by HPLC with UV detection. This method allowed the determination of HA in the range 0.5-50 mug with high reproducibility.

引用

页码：141 / 146

页数：6

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