STUDIES ON PEROXISOMES OF COLONIC MUCOSA IN CROHNS-DISEASE

被引:15
作者
AIMONEGASTIN, I
CABLE, S
KELLER, JM
BIGARD, MA
CHAMPIGNEULLE, B
GAUCHER, P
GUEANT, JL
DAUCA, M
机构
[1] UNIV NANCY 1, FAC SCI, BIOL CELLULAIRE DEV LAB, F-54506 VANDOEUVRE LES NANCY, FRANCE
[2] CHU NANCY, HOP BRABOIS, SERV MALAD APPAREIL DIGEST, F-54511 VANDOEUVRE LES NANCY, FRANCE
[3] UNIV NANCY 1, FAC MED, BIOCHIM NUTR LAB, INSERM, U308, F-54505 VANDOEUVRE LES NANCY, FRANCE
关键词
CROHNS DISEASE; INFLAMMATORY BOWEL DISEASE; COLON; HUMAN; PEROXISOMES;
D O I
10.1007/BF02090368
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The etiology and pathogenesis of Crohn's disease, a chronic inflammatory bowel pathology, have not been elucidated yet. In particular, the behavior of peroxisomes in inflamed colonic mucosa has not been investigated despite their important role in cellular oxidative metabolism. Using cytochemistry at the ultrastructural level, we have observed these catalase-positive organelles. In addition, biochemical analyses have revealed the specific activities of catalase and cyanide-insensitive acyl-CoA oxidase. Mucosal biopsy specimens from inflamed and noninflamed areas of Crohn's patients were compared to control biopsies. We found that Crohn's disease was marked by an important diminution in the peroxisomal frequency per cell unit area. If catalase activity was not affected by this pathology, cyanide-insensitive acyl-CoA oxidase, an enzyme of the peroxisomal p-oxidation system, was found diminished in inflamed and in noninflamed areas. In conclusion, our results showed that Crohn's disease is accompanied by peroxisomal modifications but the number and the enzyme activities of colonic peroxisomes are less deeply altered in Crohn's disease than during neoplasia. This fact suggests that a relation may exist between the degree of peroxisomal deficiency and the clinical severity of colonic disease.
引用
收藏
页码:2177 / 2185
页数:9
相关论文
共 48 条
[11]   EPITHELIAL PATCHY NECROSIS IN CROHNS-DISEASE [J].
DOURMASHKIN, RR ;
DAVIES, H ;
WELLS, C ;
SHAH, D ;
PRICE, A ;
OMORAIN, C ;
LEVI, J .
HUMAN PATHOLOGY, 1983, 14 (07) :643-648
[12]   ENZYMATIC AND MORPHOMETRIC EVIDENCE FOR CROHNS-DISEASE AS A DIFFUSE LESION OF GASTROINTESTINAL-TRACT [J].
DUNNE, WT ;
COOKE, WT ;
ALLAN, RN .
GUT, 1977, 18 (04) :290-294
[13]  
FRESKO D, 1982, GASTROENTEROLOGY, V82, P783
[14]   STUDIES ON CATALASE COMPARTMENTATION IN DIGITONIN-TREATED RAT HEPATOCYTES [J].
FUKAMI, MH ;
FLATMARK, T .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 889 (01) :91-94
[15]   ON THE TOPOLOGY OF THE CATALASE BIOSYNTHESIS AND BIO-DEGRADATION IN THE GUINEA-PIG LIVER - A CYTOCHEMICAL STUDY [J].
GEERTS, A ;
DEPREST, B ;
ROELS, F .
HISTOCHEMISTRY, 1984, 80 (04) :339-345
[16]  
GINZBURG L, 1956, SURGERY, V39, P347
[17]  
GREENSTEIN AJ, 1981, CANCER, V48, P2742, DOI 10.1002/1097-0142(19811215)48:12<2742::AID-CNCR2820481231>3.0.CO
[18]  
2-P
[19]  
HARVEY RF, 1980, LANCET, V1, P514
[20]  
HEMSLEY A, 1988, MOL CELL BIOCHEM, V83, P187