EPSTEIN-BARR-VIRUS (EBV) REPLICATION AND EXPRESSIONS OF EA-D (BMRF1 GENE-PRODUCT), VIRUS-SPECIFIC DEOXYRIBONUCLEASE, AND DNA-POLYMERASE IN EBV-ACTIVATED AKATA CELLS

被引:15
作者
DAIBATA, M
SAIRENJI, T
机构
[1] Department of Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655
[2] Department of Pathology, National Institute of Health, Tokyo
关键词
D O I
10.1006/viro.1993.1555
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The replication of Epstein-Barr virus (EBV) and the expression of EBV early proteins were studied in the Burkitt's lymphoma cell line Akata stimulated with anti-human immunoglobulin G antibody (anti-IgG). Akata cells contained approximately 20 copies of EBV genome per cell as covalently closed, circular DNA. EBV DNA replication was observed at 6 hr and reached a maximal level at 24 hr after treatment with anti-IgG. Virion DNA was found in the culture medium at 12 hr. The kinetics of expression of BMRF1 gene product (early antigen diffuse component; EA-D) paralleled that of EBV deoxyribonuclease (DNase) and of DNA polymerase. Immunoblotting analysis showed that three polypeptides with molecular masses of 54, 52, and 49 kilodaltons (kDa) were recognized as EA-D components. The EBV DNase polypeptide was detected by immunoblotting at 53 kDa. The anti-EBV DNA polymerase antibody recognized 120- and 54-kDa polypeptides in the Akata cells. Immunoprecipitation followed by immunoblotting showed that EA-D and EBV DNase polypeptides were coimmunoprecipitated with anti-EBV DNase antibody and with anti-EA-D monoclonal antibody. These findings indicate that EA-D forms a complex with EBV DNase polypeptide. The molecules of EA-D, EBV DNase, and DNA polymerase appear to be closely associated together on the EBV replication. © 1993 Academic Press Inc.
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页码:900 / 904
页数:5
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