MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS-SPECTROMETRY OF IMMOBILIZED DUPLEX DNA PROBES

被引：83

作者：

TANG, K

FU, DJ

KOTTER, S

COTTER, RJ

CANTOR, CR

KOSTER, H

机构：

[1] UNIV HAMBURG,DEPT BIOCHEM & MOLEC BIOL,D-20146 HAMBURG,GERMANY

[2] SEQUENOM INC,BOSTON,MA 02110

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205

[4] BOSTON UNIV,CTR ADV BIOTECHNOL,BOSTON,MA 02215

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 16期

关键词：

D O I：

10.1093/nar/23.16.3126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

引用

页码：3126 / 3131

页数：6

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