THE ERG3 GENE IN SACCHAROMYCES-CEREVISIAE IS REQUIRED FOR THE UTILIZATION OF RESPIRATORY SUBSTRATES AND IN HEME-DEFICIENT CELLS

被引：42

作者：

SMITH, SJ ^{[1
]}

PARKS, LW ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV, DEPT MICROBIOL, BOX 7615, RALEIGH, NC 27695 USA

来源：

YEAST | 1993年 / 9卷 / 11期

关键词：

SACCHAROMYCES-CEREVISIAE; STEROL; DESATURASE; ERGOSTEROL; EPISTEROL;

D O I：

10.1002/yea.320091104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ERG3 is the structural gene in Saccharomyces cerevisiae for the sterol DELTA5 desaturase that introduces the C5=6 unsaturation in ergosterol biosynthesis. The ERG3 gene has been mapped on chromosome XII, 13.7 centimorgans from GAL2 toward SPT8. The essentiality of the gene is dependent on the conditions used for the cultivation of the mutants. Insertionally inactivated mutants of ERG3 fail to grow without 'sparking' levels of DELTA5 sterols in heme-deficient cells, and are unable to grow on the respiratory substrates glycerol and ethanol.

引用

页码：1177 / 1187

页数：11

共 27 条

[1] CLONING, DISRUPTION AND SEQUENCE OF THE GENE ENCODING YEAST C-5 STEROL DESATURASE [J].

ARTHINGTON, BA ;

BENNETT, LG ;

SKATRUD, PL ;

GUYNN, CJ ;

BARBUCH, RJ ;

ULBRIGHT, CE ;

BARD, M .

GENE, 1991, 102 (01) :39-44

[2]

BECKER DM, 1991, METHOD ENZYMOL, V194, P182

[3] A POSITIVE SELECTION FOR MUTANTS LACKING OROTIDINE-5'-PHOSPHATE DECARBOXYLASE ACTIVITY IN YEAST - 5-FLUORO-OROTIC ACID RESISTANCE [J].

BOEKE, JD ;

LACROUTE, F ;

FINK, GR .

MOLECULAR & GENERAL GENETICS, 1984, 197 (02) :345-346

[4] SPECIFICITY OF DNA UPTAKE DURING WHOLE CELL-TRANSFORMATION OF SACCHAROMYCES-CEREVISIAE [J].

BRUSCHI, CV ;

COMER, AR ;

HOWE, GA .

YEAST, 1987, 3 (02) :131-137

[5] GAS-CHROMATOGRAPHIC ANALYSIS OF INTACT STERYL ESTERS IN WILD-TYPE SACCHAROMYCES-CEREVISIAE AND IN AN ESTER ACCUMULATING MUTANT [J].

FENNER, GP ;

PARKS, LW .

LIPIDS, 1989, 24 (07) :625-629

[6] THE YEAST GENE ERG6 IS REQUIRED FOR NORMAL MEMBRANE-FUNCTION BUT IS NOT ESSENTIAL FOR BIOSYNTHESIS OF THE CELL-CYCLE-SPARKING STEROL [J].

GABER, RF ;

COPPLE, DM ;

KENNEDY, BK ;

VIDAL, M ;

BARD, M .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (08) :3447-3456

[7]

GAUGHRAN J, 1992, THESIS N CAROLINA ST

[8] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[9]

Hieter P, 1990, METHODS YEAST GENETI

[10] YEAST ESCHERICHIA-COLI SHUTTLE VECTORS WITH MULTIPLE UNIQUE RESTRICTION SITES [J].

HILL, JE ;

MYERS, AM ;

KOERNER, TJ ;

TZAGOLOFF, A .

YEAST, 1986, 2 (03) :163-167

← 1 2 3 →